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Copy file name to clipboardExpand all lines: docs/instruments/core_facilities/Koch_flow.rst
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@@ -72,12 +72,12 @@ Training Details
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* If you need to do a longer sort for an experiment, you could sign up for the 1-hour assisted use, then separately sign up for more time immediately after to finish your sort.
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Sony MA-900
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-----------
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===========
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The Sony MA900 cell sorter has a `SOP <https://docs.google.com/document/d/1toqMY_qnDy0_YDkcEr2ktDJWcteKe0Pj42_scukqT5s/edit>`__ that you can follow for startup and shutdown.
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Startup
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^^^^^^^
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~~~~~~~
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You only need to start up the Sony if you want to use it over the weekend, on holidays, or outside of normal hours.
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During the week, core staff will do the start up in the morning.
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* You don’t need to include startup time when signing up to sort on weekends/holidays (e.g. don’t pay for startup time).
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Sample Prep and Sorting
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^^^^^^^^^^^^^^^^^^^^^^^
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~~~~~~~~~~~~~~~~~~~~~~~
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1. Get ice. Keep your samples on ice as much as possible after dissociation.
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2. Dissociate cells using appropriate method. Centrifuge the cells to pellet them.
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6. Add on extra time for setting up the workspace, setting voltages and gates, swapping samples, the final Bleach Cleaning and DI Rinse steps, and exporting your data.
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Shutdown
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^^^^^^^^
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~~~~~~~~
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Follow the shutdown SOP, with the exception that on step 7 ("Turn off the air compressor and blue switch on the air-line"),
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do **not** turn off the blue switch on the air-line,
Copy file name to clipboardExpand all lines: docs/protocols/tc/virus/viral_titer.rst
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@@ -98,7 +98,7 @@ Note that individual cells with detectable expression may be contain one or more
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First, let's define the **multiplicity of infection (MOI)**, :math:`\lambda`, as the ratio of viral transducing units (TU) to number of cells, *n*:
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.. math:: \lambda = \frac{\text{# TU}}{n}
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.. math:: \lambda = \frac{\text{number of TU}}{n}
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We can obtain a particular MOI in an experiment by using a calculated **volume of virus**, *v*, if we know the **viral titer**, *t*:
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cells with at least one transduction:
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.. math::
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\begin{align}
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P(x>0) &= 1 - P(x=0) \\
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&= 1 - \frac{\lambda^0 e^{-\lambda}}{0!} \\
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&= 1 - e^{-\lambda} \\
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&= 1 - e^{v / n \ \times\ t}
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\end{align}
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Thus, the flow cytometry data from the experiment above can give us a fraction of expressing cells, which we can use with known values of *v* and *n* (experimental parameters)
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to find *t*, the viral titer. To make this calculation more accurate, we can measure the fraction of expressing cells for several different volumes of virus---exactly the
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