diff --git a/Sources/Rendering/Core/Glyph3DMapper/test/testGlyph3DMapperShiftScale.js b/Sources/Rendering/Core/Glyph3DMapper/test/testGlyph3DMapperShiftScale.js
new file mode 100644
index 00000000000..ac33024eed5
--- /dev/null
+++ b/Sources/Rendering/Core/Glyph3DMapper/test/testGlyph3DMapperShiftScale.js
@@ -0,0 +1,98 @@
+import test from 'tape';
+import testUtils from 'vtk.js/Sources/Testing/testUtils';
+
+import 'vtk.js/Sources/Rendering/Misc/RenderingAPIs';
+import vtkActor from 'vtk.js/Sources/Rendering/Core/Actor';
+import vtkCellArray from 'vtk.js/Sources/Common/Core/CellArray';
+import vtkGlyph3DMapper from 'vtk.js/Sources/Rendering/Core/Glyph3DMapper';
+import vtkPolyData from 'vtk.js/Sources/Common/DataModel/PolyData';
+import vtkRenderWindow from 'vtk.js/Sources/Rendering/Core/RenderWindow';
+import vtkRenderer from 'vtk.js/Sources/Rendering/Core/Renderer';
+
+function makePolyDataFromPoints(coords) {
+  const polyData = vtkPolyData.newInstance();
+  polyData.getPoints().setData(Float32Array.from(coords), 3);
+  return polyData;
+}
+
+function updatePoints(polyData, coords) {
+  polyData.getPoints().setData(Float32Array.from(coords), 3);
+  polyData.getPoints().modified();
+  polyData.modified();
+}
+
+function makeLineGlyphSource() {
+  const source = makePolyDataFromPoints([-0.5, 0, 0, 0.5, 0, 0]);
+  source.setLines(
+    vtkCellArray.newInstance({
+      values: new Uint32Array([2, 0, 1]),
+    })
+  );
+  return source;
+}
+
+function getActivePrimitiveCABOs(openGLRenderWindow, mapper) {
+  const openGLMapper = openGLRenderWindow.getViewNodeFor(mapper);
+  const primitives = openGLMapper.getReferenceByName('primitives');
+  return primitives
+    .map((primitive) => primitive.getCABO())
+    .filter((cabo) => cabo.getElementCount() > 0);
+}
+
+test.onlyIfWebGL(
+  'Test vtkGlyph3DMapper clears source VBO shift/scale after shifted glyph centers',
+  (t) => {
+    const gc = testUtils.createGarbageCollector(t);
+
+    const container = document.querySelector('body');
+    const renderWindowContainer = gc.registerDOMElement(
+      document.createElement('div')
+    );
+    container.appendChild(renderWindowContainer);
+
+    const renderWindow = gc.registerResource(vtkRenderWindow.newInstance());
+    const renderer = gc.registerResource(vtkRenderer.newInstance());
+    renderWindow.addRenderer(renderer);
+
+    const centers = gc.registerResource(
+      makePolyDataFromPoints([10000000, 0, 0, 10000001, 0, 0])
+    );
+    const source = gc.registerResource(makeLineGlyphSource());
+    const mapper = gc.registerResource(vtkGlyph3DMapper.newInstance());
+    const actor = gc.registerResource(vtkActor.newInstance());
+
+    mapper.setInputData(centers, 0);
+    mapper.setInputData(source, 1);
+    mapper.setScalarVisibility(false);
+
+    actor.setMapper(mapper);
+    renderer.addActor(actor);
+
+    const openGLRenderWindow = gc.registerResource(
+      renderWindow.newAPISpecificView()
+    );
+    openGLRenderWindow.setContainer(renderWindowContainer);
+    renderWindow.addView(openGLRenderWindow);
+    openGLRenderWindow.setSize(1, 1);
+
+    renderWindow.render();
+
+    const shiftedCABOs = getActivePrimitiveCABOs(openGLRenderWindow, mapper);
+    t.ok(shiftedCABOs.length > 0, 'glyph source VBOs were built');
+    t.ok(
+      shiftedCABOs.some((cabo) => cabo.getCoordShiftAndScaleEnabled()),
+      'far glyph centers enable shift/scale on source VBOs'
+    );
+
+    updatePoints(centers, [-0.5, 0, 0, 0.5, 0, 0]);
+    renderWindow.render();
+
+    const unshiftedCABOs = getActivePrimitiveCABOs(openGLRenderWindow, mapper);
+    t.ok(
+      unshiftedCABOs.every((cabo) => !cabo.getCoordShiftAndScaleEnabled()),
+      'source VBO shift/scale is cleared once glyph centers no longer use it'
+    );
+
+    gc.releaseResources();
+  }
+);
diff --git a/Sources/Rendering/OpenGL/Glyph3DMapper/index.js b/Sources/Rendering/OpenGL/Glyph3DMapper/index.js
index ae1205c333a..8c1c0776310 100644
--- a/Sources/Rendering/OpenGL/Glyph3DMapper/index.js
+++ b/Sources/Rendering/OpenGL/Glyph3DMapper/index.js
@@ -754,14 +754,16 @@ function vtkOpenGLGlyph3DMapper(publicAPI, model) {
 
     superClass.buildBufferObjects(ren, actor);
 
-    // apply shift + scale to primitives AFTER vtkOpenGLPolyDataMapper.buildBufferObjects
-    // so that the Glyph3DMapper gets the last say in the shift + scale
-    if (useShiftAndScale) {
-      for (let i = primTypes.Start; i < primTypes.End; i++) {
-        model.primitives[i]
-          .getCABO()
-          .setCoordShiftAndScale(coordShift, coordScale);
-      }
+    // Apply shift + scale to primitives AFTER vtkOpenGLPolyDataMapper.buildBufferObjects
+    // so that the Glyph3DMapper gets the last say in the shift + scale. Clear
+    // the previous frame's transform when the current glyph centers no longer
+    // need it; otherwise glyphs keep rendering with stale source-VBO scaling.
+    const sourceCoordShift = useShiftAndScale ? coordShift : null;
+    const sourceCoordScale = useShiftAndScale ? coordScale : null;
+    for (let i = primTypes.Start; i < primTypes.End; i++) {
+      model.primitives[i]
+        .getCABO()
+        .setCoordShiftAndScale(sourceCoordShift, sourceCoordScale);
     }
   };
 }